ABSTRACT
Objective: To establish and evaluate a rapid nucleic acid detection method for SARS-CoV-2 based on COYOTE® Flash20 real-time fluorescent quantitative PCR instrument. Methods: A rapid reaction system was constructed by using specific primer and probe sets targeting ORF1ab and N gene of SARS-CoV-2, and the sensitivity and specificity of the system were verified. At the same time, 108 clinical samples of COVID-19 were used to evaluate the application of this method. Results: The detection method did not require nucleic acid extraction, and the manual operation time was only one minute. After the sample was sent to the system, the test could be completed in 30 minutes. The detection limit of this method was 4×102 copies/ml. It had no cross-reactivity with other human coronaviruses (including HCoV-229E, HCoV-NL63, HCoV-OC43, HCoV-HKU1, SARS-CoV and MERS-CoV) and other respiratory viruses. The evaluation of clinical sample application showed that the total coincidence rate with the conventional RT-qPCR which required nucleic acid extraction was 98.15%. Conclusions: Through the application evaluation of the rapid fluorescent quantitative PCR method of SARS-CoV-2, it was found that the method was simple, fast, specific and sensitive, and it was suitable for real-time and rapid detection needs in varieties of situations. Copyright © 2021 by the Chinese Medical Association.
ABSTRACT
With the outbreak and spread of 2019 novel coronavirus (2019-nCoV, or SARS-CoV-2) infection in Wuhan, China, the number of children infected has also increased significantly, and even severe and critically ill children have appeared. In order to curb the spread of the epidemic, it is important to understand the clinical manifestations of 2019-nCoV infection in children and strengthen the key points for family prevention and control. According to the diagnostic standards and protective measures issued by the National Health Commission, this article summarizes the epidemiological characteristics and clinical manifestations of some child cases, and proposes to focus on the clinical manifestations, treatment procedures, and family protection and daily health care. © 2020, Editorial Board of Medical Journal of Wuhan University. All right reserved.